In one scene snakes and other reptiles are seen in a laboratory where Poison Ivy works. When she is on the ground, a few small snakes are seen crawling around her. This scene was shot in cuts. Fake and real snakes were used. Real snakes were used for the establishing shots of the reptiles in their containers and on the ground. Fake snakes and reptiles were used for the scenes where a few containers are seen falling to the ground. The trainer placed the animals in their containers and retrieved them after the establishing shot.

When Mr. Freeze is terrorizing the city with his freezing gun, a dog is seen lifting his leg on a fire hydrant. While relieving himself, the dog is frozen by Mr. Freeze. Later, Batman thaws everyone out and the dog is seen shaking his body and then walks away. For this scene a real and a fake dog were used. Water was sprayed on the ground along with dry ice 4 feet behind the dog. The trainer placed the dog on his mark and applied two handfuls of crushed ice on his back and head, then cued the dog to shake. The dog walked to his trainer out of camera range and was immediately dried with towels. A fake frozen dog was used for the shot of the dog after Mr. Freeze zapped him with his freezing ray.