All grounds were inspected for hazards and sets were closed off and secured. Cast and crew members were introduced to the animals and instructed on their proper handling.

Alligator

For the scene in which some men pin down an alligator in a motel parking lot, tie it up and place it in a truck bed, costumed trainers performed the action. They had extensive experience with this trained alligator and took special care in handling it gently.

Horses

For the scene in which two police cars chase a horse down a road at night, the horse and stunt drivers were very well-rehearsed. A lead horse ran ahead to cue the other horse to chase after it —the two horses knew each other and are part of a performing drill team. The horse happily ran down the street while the stunt drivers kept their cars at a safe distance. The horse was retrieved right after filming.

Deer

For the scene in which a deer is hit by a car, the "live" deer was animatronic. A prop was used for dead deer.

Other

Due to late notification or limited resources, American Humane did not monitor any of the snake action.

Although The Yellow Handkerchief uses the language American Humane monitored the animal action at the end of the film, American Humane's Film and TV Unit did not issue an official disclaimer to the film before the end credits were completed. This production did not give American Humane a pre-release screening of the film, which is one of the requirements for issuing the disclaimer. However, an American Humane Post-Production staff member screened the film in the theater after it had opened and ultimately deemed the film worthy of an American Humane disclaimer, which should read, "American Humane monitored some of the animal action. No animals were harmed in those scenes.